Thermometer Calibration Information and Measuring pH of Meat

Thermometer Calibration

HACCP based food safety programs require accurate record keeping to be successful. Temperature is often the parameter of interest when monitoring a critical control point (CCP). To assure that a temperature dependant process is under control a calibrated thermometer must be used to record temperatures. The majority of thermometers can be calibrated following a few basic procedures.

To be considered accurate, a thermometer must be calibrated to measure within +/- 2° F (1.1° C) of the actual temperature. Actual temperature can be determined in a variety of ways including measurement with an NIST (National Institute of Standards and Technology) certified reference thermometer or simply through using an ice water solution or boiling water. Another option is the use of sophisticated, and often high cost, calibration equipment that is increasingly becoming available commercially.

The simplest and cheapest way to calibrate a thermometer is through either the use of ice water or boiling water. Distilled water should always be used as dissolved solutes in tap water can significantly affect both freezing and melting points. Another important consideration is the altitude (Table 1) at which calibration is being performed. At sea level, pure water boils at 212° F but at 10,000 feet above sea level it boils at only 194° F. Barometric pressure also has an effect on boiling point but the effect is much less than that of altitude.

You may visit to determine the altitude of your city.

Thermometers intended for measuring higher temperature items, such as cooked product, should be calibrated in boiling water while those used for taking lower temperatures should be calibrated in ice water. When calibrating in ice water both the water and ice should be composed of distilled water. In either case care should be taken to prevent the thermometer from contacting the container being used as this could result in erroneous temperature readings.

Calibration in Ice Water

  1. Add crushed ice and distilled water to a clean container to form a watery slush.
  2. Place thermometer probe into slush for at least one minute taking care to not let the probe contact the container.
  3. If the thermometer does not read between 30° and 34° F adjust to 32° F. Non-adjustable thermometers should be removed from use until they have been professionally serviced.

Calibration in Boiling Water

  1. Bring a clean container of distilled water to a rolling boil.
  2. Place thermometer probe into boiling water for at least one minute taking care not to let the probe contact the container.
  3. If the thermometer does not read between 210° and 214° F adjust to 212° F. Non-adjustable thermometers should be removed from use until they have been professionally serviced.

Thermometers that are found to be inaccurate (i.e. do not measure within +/- 2°F of the actual temperature) should either be manually adjusted or serviced by a professional. Thermometers that have a history of deviating from actual temperature measurements should be discarded and replaced. To assure accuracy, NIST certified thermometers must be re-certified annually.

Thermometers that cannot be easily calibrated through direct immersion in boiling or ice water can be calibrated by comparing readings with another calibrated thermometer. Thermometers that may be calibrated in this way include smokehouse probes and room temperature thermometers. When doing this it is important that the thermometer used for the comparison has been calibrated recently. All thermometers should be calibrated regularly with those used for monitoring CCP's being calibrated either daily or weekly, depending on the volume of your operations. Any thermometer that has been subjected to abuse, such as being dropped on the floor, should be immediately recalibrated to assure accuracy. Hard to calibrate thermometers could be compared directly with NIST reference thermometers but this may be undesirable as many of these reference thermometers are glass and mercury and could present chemical and physical hazards in food production areas.

Table 1 - Relationship of Altitude to Boiling Point of Pure Water

Feet Above Sea Level Boiling Point Feet Above Sea Level Boiling Point
212° F
203° F
211° F
203° F
210° F
201° F
209° F
199° F
208° F
197° F
207° F
194° F
206° F
190° F
205° F
187° F
204° F

Measuring pH of Meat

Fermented Sausages and Post-rigor Muscle

  1. Calibrate the pH meter using pH 7 and pH 4 standardization buffers.
  2. Cut meat sample into small pieces and weight approximatley 10 grams into a blender cup. Run duplicates on each sample.
  3. Add 100 ml of distilled deionized water
  4. Blend for 30 seconds on high speed.
  5. Transfer sample to a beaker. Read the pH as soon as possible.
  6. Add a stir bar to the beaker, turn on the stir plate and place the pH electrode in the sample. Wait for the ready light to come on before recording the pH value.
  7. Blender cups, beakers and stir bars can be rinsed in distilled water between samples. The pH electrode should be rinsed with distilled water between each sample and periodically rinsed with acetone from a squeeze bottle to remove fat buildup. (Read manufactures directions on care of the pH electrode before using this procedure).